No. 95 CAMAG Bibliography Service "yellow pages", Section 32

32. Pharmaceutical and biomedical applications

95 049
S. B. AGARWAL, N. D. GRAMPUROHIT*, A. S. PAREKAR (*C. U. Shah College of Pharmacy, S.N.D.T. Women's University, Santacruz (W), Mumbai 4

00 049
, India): Standardization of herbal formulations containing kurchi (Holarrhena antidysenterica). IPC 56th 2004, Abstract No. D-9. HPTLC for the standardization of the alkaloid conessine in several market formulations containing kurchi bark, on silica gel with toluene - methanol - chloroform 1:2:1. Detection by spraying with Dragendorff's reagent. Densitometric evaluation at 460 nm. The method was validated for accuracy, precision, linearity range, specificity, LOD, LOQ and found suitable for routine analysis of herbal formulations containing Kurchi as main ingredient. Pharmaceutical research, quality control, densitometry, comparison of methods, postchromatographic derivatization, quantitative analysis, conessine 32a

95 050
Danica AGBABA*, D. NOVOVIC, K. KARLJIKOVIC-RAJIC, V. MARINKOVIC (*Institute of Pharmaceutical Chemistry and Drug Analysis, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, P. O. Box 146, 11000 Serbia and Montenegro): Densitometric determination of omeprazole, pantoprazole, and their impurities in pharmaceuticals. J. Planar Chromatogr. 17, 169-172 (2004). HPTLC of omeprazole and pantoprazole and their impurities omeprazole sulfone and N-methylpantoprazole on silica gel with chloroform - 2-propanol - 25 % ammonia - acetonitrile (108:12:3:40). Detection under UV light at 254 nm. Quantitation of omeprazole and omeprazole sulfone at 300 nm and of pantoprazole and N-methylpantoprazole at 295 nm in re- flectance-absorbance mode. Regression coefficients (r >0.998), recovery (90.7 - 120.0 %), and detection limits (0.025 - 0.05 %) were validated and found to be satisfactory. Quality control, HPTLC, densitometry, quantitative analysis, omeprazole, pantoprazole 32a

95 053
Mugdha BHOSALE*, A. R. PARADKAR, K. R. MAHADIK, K.S. JAIN (*Sinhgad College of Pharmacy, Vadgaon (Bk), Pune 4

11 041
, India): Stability indicating HPTLC determination of cefuroxime axetil as bulk drug and in pharmaceutical formulations. 56th IPC 2004, Abstract No. GP-46. Stability indicating HPTLC determination of cefuroxime axetil in bulk drug and in formulations on silica gel with chloroform Ð methanol 23:2. Quantitative determination by scanning at 278 nm. The sample was subjected to acidic, and alkali hydrolysis, oxidation and photo degradation. The degraded products were well separated. The method was validated for accuracy, precision, linearity, specificity, ruggedness, and recovery (98Ð100 %). Pharmaceutical research, quality control, quantitative analysis, densitometry, postchromatographic derivatization, comparison of methods, HPTLC, cefuroxime axetil 32a

95 055
Mira CAKAR*, G. POPOVIC, S. VLADIMIROV (*Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, P. O. Box 146, 11000 Belgrade, Serbia and Montenegro): Simultaneous HPTLC determination of imidazole antimycotics and parabens in creams. J. Planar Chromatogr. 17, 177-180 (2004). HPTLC of bifonazole, econazole nitrate, methyl- and propylparaben on silica gel with ethyl acetate - n-hexane - methanol - ammonia - diethylamine 5:40:8:4:20 in a twintrough chamber. Quantitation by scanning in reflectance/absorbance mode at 230 nm (econazole nitrate), 250 nm (bifonazole), and 300 nm (parabens). Quality control, densitometry, HPTLC, quantitative analysis, qualitative identification, antimycotics, parabens 32a

95 083
M. S. CHARDE*, M. J. UMEKAR, S. B. JOSHI, A. V. KASTURE (*Department of Pharmaceutical Sciences, Nagpur University, Nagpur 440033, India): Estimation of ranitidine HCl and domperidone in combined dosage form using HPTLC. IPC 56th 2004, Abstract No. GP-8. Simultaneous HPTLC determination of ranitidine and domperidone on silica gel with methanol - 1, 4-dioxan 2:3. Quantitative determination by densitometric scanning at 282 nm. Rf values were 0.33 for ranitidine and 0.78 for domperidone. Linearity range was 0.5 - 2.5 mg/mL for both of the drugs. The recovery was in the range of 100.25 - 100.78 %. The method is suitable for the analysis of both drugs in combined dosage form. Pharmaceutical research, quality control, quantitative analysis, densitometry, comparison of methods, postchromatographic derivatization, HPTLC, ranitidine, domperidone 32a

95 059
Shruti DHURU*, Pratima TATKE, K. K. SINGH (*C.U.Shah College of Pharmacy, S.N.D.T. Women's University, Santacruz (West) , Mumbai 4

00 049
, India): Standardization and evaluation of Neem oil in pharmaceutical formulations by HPTLC. IPC 56th 2004, Abstract No. G-28. Neem Oil obtained from the seed kernels of Azardirachla indica (Meliaceae) is a fixed oil known as oil of Margosa. An HPTLC method is reported for the analysis of Neem oil as a bulk drug and formulations containing oil. TLC of neem oil extracted with chloroform, on silica gel with chloroform - n-hexane - methanol 18:2:1. Quantitative determination by scanning at 254 nm. The linearity range was 100 - 500 mg/mL. Formulations were found to contain 0.35 g/g of Neem Oil. Pharmaceutical research, quality control, quantitative analysis, densitometry, comparison of methods, postchromatographic derivatization, HPTLC, neem oil 32a

95 051
S. B. GAICA, D. M. OPSENICA, B. A. SOLAJA, Z. L. TESIC, Dusanka M. MILOJKOVIC-OPSENICA* (*Faculty of Chemistry, University of Belgrade, P. O. Box 158, 11001 Belgrade, Serbia and Montenegro): The effect of the structure of mixed tetraoxanes on their chromatographic behavior on different adsorbents. J. Planar Chromatogr. 17, 342-349 (2004). TLC of 29 1,2,3,4- tetraoxanes on silica gel, cyano phase, and RP-18. The binary mobile phases ethyl acetate - petroleum ether and ethyl acetate - toluene were used under normal-phase conditions, and water - organic modifier (methanol, acetone, dioxane) under reversed-phase conditions. Chromatography was performed using a HPTLC horizontal developing chamber equilibrated for 15 min with the vapor of the mobile phase. Detection by spraying with 50 % sulfuric acid and heating until the spots became visible. Pharmaceutical research, qualitative identification 32a

95 061
M. GANDHIMATHI, S. C. VIJAY KUMAR*, T. K. RAVI, Shaise JACOB, Lekha MATHEW, S. MALATHI (*Department of Pharmaceutical Analysis, College of Pharmacy, SRIPMS,395, Sa rojini Naidu Road, Coimbatore 614044, India): Simultaneous estimation of Loratadine and Ambroxol from formulation by HPTLC. IPC 56th 2004, Abstract No. G-20. Simultaneous HPTLC determination of loratadine and ambroxol in combined dosage form on silica gel with n-hexane - dichloromethane - triethanolamine 11:8:1.The Rf value of loratadine and ambroxal was found to be 0.40 and 0.16 respectively. Quantitative evaluation by scanning at 254 nm. The method was linear in the range of 0.2 -1 mg/spot for loratadine and 1.2 - 6 mg/spot for ambroxol with recovery of 98.2 - 98.5 %. The method was validated for accuracy, precision, linearity, specificity, LOD, and LOQ. Pharmaceutical research, quality control, densitometry, comparison of methods, postchromatographic derivatization, quantitative analysis, HPTLC, loratadine, ambroxol 32a

95 082
K. R. GUPTA*, A. N. MALIYE, M. R. TAJNE, S. G. WADODKAR (*Department of Pharmaceutical Sciences, Nagpur 440033, India): Stability indicating HPTLC determination of indapamide in tablets. IPC 56th 2004, Abstract No. GP-11. Stability indicating HPTLC determination of indapamide in tablets on silica gel with toluene - methanol 7:3. Quantitative determination by scanning at 246 nm. Optimization of experimental parameter such as band size, chamber saturation, and slit width. The method was linear in the range of 1.4 - 3.72 g, recovery was 100.01 %. The drug was subjected to stress conditions according to ICH guidelines, degradation products were separated from the pure drug. The method was validated for accuracy, precision, linearity, and specificity. Pharmaceutical research, quality control, quantitative analysis, densitometry, postchromatographic derivatization, comparison of methods, HPTLC, indapamide 32a

95 066
H. HOPKALA, A. POMYKALSKI* (*Department of Medicinal Chemistry, Faculty of Pharmacy, Prof. Skubiszewski Medical Universit y of Lublin, 6 Chodzki St., 20-093 Lublin, Poland): TLC analysis of non-steroidal anti-inflammatory drugs and videodensitometric determination of fenbufen in tablets. J. Planar Chromatogr. 17, 383-387 (2004). TLC of fenbufen, ibuprofen, ketoprofen, diclofenac sodium, mefenamic acid, and tiaprofenic acid on silica gel by ascending and horizontal techniques, and on RP-18 in horizontal chambers. Good separation was achieved on silica gel by horizontal development with chloroform - methanol - 25 % ammonia 67:25:8; reversed phase chromatography on RP-18 with 0.15 mol/L phosphate buffer, pH 5.73 - 10 % CTMA-Br (N-cetyl-N,N,N-trimethylammonium bromide) in methanol 7:13 enabled better separation of the six drugs. Detection under UV light at 254 nm - for ibuprofen detection was best achieved after normal phase chromatography with 20 % aqueous sodium carbonate solution. A simple videodensitometric procedure was developed and validated. RSD for quantitation of fenbufen was 2.44 - 3.10 %. Quality control, densitometry, quantitative analysis, non-steroidal anti-inflammatory drugs 32a

95 068
A. JASHIDI (Department of Novel Drug-delivery Systems, Iran Polymer and Petrochemical Institute, P. O. Box 14185/458, Tehran, Iran): A convenient and high throughput HPTLC method for determination of progesterone in release media of silicon-based controlled-release drug-delivery systems. J. Planar Chromatogr. 17, 229-232 (2004). HPTLC of progesterone on silica gel in an automatic multiple development chamber (AMD) with toluene - 2-propanol 9:1 without chamber saturation and with 10 min drying time. Visual inspection under UV light at 254 nm. Quantitative determination in reflectance mode at 252 nm. Limits of quantitation and detection were 25 and 5 ng/zone. Quality control, AMD, HPTLC, densitometry, quantitative analysis, progesterone 32a

95 069
N.S. JEGANATHAN*, M. RAJ MOHMED, R.MANAVALAN (*Dept.of Pharmacy, Annamalai University, Annamalai Nagar -608002 TN, India): Quantitative determination of piperine in Trikatukuc Curanam by HPTLC. IPC 56th 2004, Abstract No. DP-33. An HPTLC method is reported for the standardization of Trikatukuc Curanam, an Ayurvedic preparation with Piper nigrum and Piper longum, both containing piperine as major alkaloid. HPTLC of piperine on silica gel with toluene - ethyl acetate 7:3. Extraction with methylene chloride, the evaporated residue of the organic layer was taken in ethyl acetate and subjected to the analysis. The band corresponding to piperine was scanned at 338 nm. Linearity was 8-40 ng with recovery of 99.03 %. Pharmaceutical research, quality control, postchromatographic derivatization, densitometry comparison of methods, quantitative analysis, piperine 32a

95 003
H. KALÁSZ et al., see section 2a

95 070
N. KAUL, H. AGRAWAL, A. R. PARADKAR, K. R. MAHADIK* (*Department of Quality Assurance Techniques, Bharati Vidyapeeth Deemed University, Poona College of Pharmacy, Erandwane, Pune-411038, Maharashtra State, India): Stability-indicating high-performance thin-layer chromatographic determination of zidovudine as the bulk drug and in pharmaceutical dosage forms. J. Planar Chromatogr. 17, 264-274 (2004). HPTLC of zidovudine (3'-azido-3'-deoxythymidine) and degradation products on silica gel with toluene - carbon tetrachloride - methanol - acetone 35:35:20:1. Quantitative determination by absorbance measurement at 270 nm. The method was validated for precision, robustness, and recovery. Limit of detection was 20 ng per spot, limit of quantitation 40 ng. HPTLC, densitometry, quantitative analysis, zidovudine 32a

95 071
Amandeep KAUR*, Prateek K. JAIN and R. K. AGRAWAL (*Pharmaceutical Chemistry, Research Laboratory, Department of Pharmaceutical Science, Dr. Hari Singh gour University Sagar m.p. 470003, India): TLC densitometric method for the quantification of conessine in Holarrhena antidysenterica. IPC 56th 2004, Abstract No. G-5. HPTLC of conessine in Holarrhena antidysentrica, an important ayurvedic drug, on silica gel with toluene - ethyl acetate - diethyl amine 13:5:2. Detection by spraying with Dragendorff's reagent. Quantitative determination by densitometric scanning at 520 nm. Different market samples of the drug were found to contain 0.30 - 1.46 % of conessine with recovery of 95.18 - 102.70 % Pharmaceutical research, quality control, densitometry, comparison of methods, postchromatographic derivatization, quantitative analysis, HPTLC, conessine 32a

95 060
M. G. PAI*, Dattesh VEREKAR, K. VENKATESHWAR RAO (*Goa College of Pharmacy, Panaji, Goa, India): Development and validation of a new sensitive method for the simultaneous estimation of amlodipine - atenolol in tablets by HPTLC. IPC 56th 2004, Abstract No. GP-5. Simultaneous HPTLC determination of amlodipine and atenolol on silica gel with ethyl acetate - methanol - ammonia 60:40:3. Quantitative determination by scanning at 254 nm. The method was found linear in the range of 0.5 - 5.0 mg/mL amlodipine and 5.0 mg - 50 mg/mL atenolol. Recovery was 98.11 - 101.5 % for both of the compounds. The method was validated for accuracy, precision, linearity, specificity, LOD, and LOQ. Pharmaceutical research, quality control, quantitative analysis, postchromatographic derivatization, comparison of methods, densitometry, HPTLC, amlodipine, atenolol 32a

95 078
R. PIETRAS*, H. HOPKALA, D. KOWALCZUK, A. MALYSZA (*Department of Medicinal Chemistry, Medical University, Chodzki 6, 20-093 Lublin, Poland): Normal-phase TLC separation of some antiarrhythmics. Densitometric determination of mexiletine hydrochloride in capsules. J. Planar Chromatogr. 17, 213-217 (2004). TLC of disopyramide, flecainide, mexiletine, tocainide, and verapamil on aluminium oxide and silica gel in horizontal chambers. The best mobile phase for separation on the alumina plates was tetrahydrofuran - hexane - 25 % ammonia 25:24:1 and on silica chloroform - tetrahydrofuran - ethanol - 25 % ammonia 81:19:20:1. Detection under UV light at 210 nm and by use of different reagents. Quantification of mexiletine hydrochloride in capsules was performed densitometrically at 254 nm. Correlation coefficient in the concentration range 20 - 45 µg per band was 0.9974, with RSD of 5.23 %. Quality control, quantitative analysis, densitometry, antiarrhythmic drugs 32a

95 080
P. N. PRESANNAKUMARAN, Ann Mary ISAAC* (*Thejus Tharu. College of Pharmaceutical Sciences, Medical College, Trivandrum, India): Estimation of rabeprazole using HPTLC. 56th IPC 2004, Abstract No. GP-48. HPTLC of rabeprazole sodium in tablet dosage form on silica gel with ethyl acetate - methanol 9:1. Optimization of experimental parameters such as bandwidth, chamber saturation time, solvent front migration, and mobile phase composition. Quantitative determination by scanning at 260 nm. The Rf value was 0.59. The method was linear with a correlation coefficient of 0.99, recovery was 98.81 %. Pharmaceutical research, quality control, comparison of methods, postchromatographic derivatization, quantitative analysis, densitometry, HPTLC, rabeprazole 32a

95 081
Alina PYKA (Department of Analytical Chemistry, Faculty of Pharmacy, Silesian Academy of Medicine, 4, Jagillonska Street, PL-41200 Sosnowiec, Poland): Study of lipophilicity and application of selected topological indexes in QSAR analysis of nicotinic acid derivatives. Part I. J. Planar Chromatogr. 17, 275-279 (2004). HPTLC of nicotinic acid and selected derivatives (methyl nicotinate, ethyl nicotinate, isopropyl nicotinate, butyl nicotinate, hexyl nicotinate, benzyl nicotinate, nicotinamide, N-methyl nicotinamide) on RP-18 with methanol - water in different volume proportions after chamber saturation for 30 min. Detection under UV light at 254 nm. Investigation of the lipophilicity by TLC and use of the data for quantitative structure-activity relationships. Pharmaceutical research, HPTLC, qualitative identification, quantitative structure-activity relationships 32a

95 079
T. K. RAVI, Prabhathi KITANIA, M. GANDHIMATHI, P. RAVIMATHI*, Satheesh KUMAR N. (*Department of Pharmaceutical Analysis, College of Pharmacy, SRIPMS, 395, Sarojini Naidu Road, Coimbatore 6

41 044
, India): HPTLC method for the estimation of mirtazapine from tablet formulation. IPC 56th 2004, Abstract No. GP-17. HPTLC of mirtazapine in tablet dosage form on silica gel with chloroform - methanol 1:9. The Rf value was 0.50 - 0.52, the linearity range was 0.3 - 1.5 mg/spot. Quantitative determination by scanning at 295 nm. The method was validated for accuracy, precision, linearity, specificity, LOD, and LOQ. Pharmaceutical research, quality control, comparison of methods, postchromatographic derivatization, quantitative analysis, densitometry, HPTLC, mirtazapine 32a

95 105
M. SAJEWICZ et al., see section 38

95 091
R. T. SANE, S. N. MENON, M. MOTE*, S. INAMDAR, A. MENEZES (*TDM laboratories, Plot No. 194, Scheme No. 15, Road No. 15, Sion (E), Koliwada, Mumbai-22, India): Highperformance thin-layer chromatographic determination of aceclofenac in the bulk drug and in pharmaceutical preparations. J. Planar Chromatogr. 17, 238-240 (2004). HPTLC of aceclofenac and mosapride citrate (as internal standard) on silica gel in a twin-trough chamber equilibrated with the mobile phase with toluene - methanol - ethyl acetate - glacial acetic acid 550:250:200:1. Quantitative determination by densitometry at 284 nm. Quality control, densitometry, HPTLC, quantitative analysis, aceclofenac 32a

95 085
Sapna SHRIKUMAR, A. SAIT, A. JITENDRA*, M. SUKUMAR, T. K. RAVI (*Department of Pharmaceutical Analysis, College of Pharmacy, SRIPMS, Coimbatore-614044, India): An HPTLC method for the standardization of Curculigo Orchioides and its formulations for antioxidant activity using gallic acid as standard. IPC 56th 2004, Abstract No. G-18. Curculigo Orchioides (Amary llidaceae) is used in various ayurvedic formulations. The rhizomes contain about 5.78 % total phenolis, gallic acid being the major component of the alcoholic extracts. HPTLC of gallic acid on silica gel with toluene - ethyl acetate - glacial acetic acid 25:15:1. The Rf value of gallic acid was 0.19, the linearity range of 150-750 ng/spot. Rhizomes were found to contain 2.54 % gallic acid, formulations contained 5.13 % gallic acid, recovery was 99.5 %. Pharmaceutical research, quality control, densitometry, comparison of methods, postchromatographic derivatization, quantitative analysis, HPTLC, Curculigo Orchioides, gallic acid 32a

95 086
Sapna SHRIKUMAR, A. SAIT, Manju GOPI*, A. SUGANTHI, M. SUKUMAR, T. K. RAVI (*Department of Pharmaceutical Analysis, College of Pharmacy, SRIPMS, Coimbatore 641 044, India): HPTLC method for the standardization of Aphanamixis Polystachya for its antioxidant activity using gallic acid as standard. 56th IPC 2004, Abstract No. GP-36. HPTLC for the standardization of gallic acid in alcoholic extracts of Aphanamixis polystachya (Meliceae) on silica gel with toluene - ethyl acetate - formic acid - methanol 15:15:4:1. Rf value of gallic acid was 0.45, linearity was 15 - 75 mg/mL. Formulations were found to contain 9.56 % of gallic acid. Gallic acid is the main phenolic compound and can be used for standardization of the crude drug. Pharmaceutical research, quality control, quantitative analysis, densitometry, comparison of methods, postchromatographic derivatization, HPTLC, Aphanamixis polystachya, gallic acid 32a

95 087
Sapna SHRIKUMAR*, S. CICY, M. SUKUMAR, T. K. RAVI (*Department of Pharmaceutical Analysis, College of Pharmacy, SRIPMS, Coimbatore 6

41 044
, India): HPTLC method for the estimation and quantification of gallic acid in some ayurvedic formulations of triphala. IPC 56th 2004, Abstract No. GP-26. HPTLC of triphala, an ayurvedic formulation containing about 3.60 % of total phenolics. Separation of alcoholic triphala extracts on silica gel with n-hexane - ethyl acetate 2:1. Rf value of the main spot gallic acid was 0.04 in triphala and its formulation. The method was found to be very specific for gallic acid having a linearity range of 0.2 - 1.6 mg/mL. Several formulations analyzed by HPTLC contained 5.2 - 7.6 % of gallic acid. The reported method is suitable for estimation of gallic acid in raw material and formulations. Pharmaceutical research, quality control, quantitative analysis, densitometry, comparison of methods, postchromatographic derivatization, HPTLC, triphala 32a

95 088
R. SKIBINSKI, Genowefa MISZTAL* (*Department of Medicinal Chemistry, Medical University of Lublin): Determination of fluvoxamine and moclobemide in tablets by densitometric and videodensitometric TLC. J. Planar Chromatogr. 17, 224-228 (2004). TLC of fluvoxamine and moclobemide on silica gel in horizontal chambers with benzene - acetone - ethanol - 25 % ammonia 9:7:2:1. Densitometric detection and quantification were performed at 249 nm and 236 nm, respectively. The range of linearity was 1 - 10 µg per spot; the RSD was less than 2.5 % for densitometry and less than 5.1 % for videodensitometry. Quality control, densitometry, quantitative analysis, fluvoxamine, moclobemide 32a

95 009
B. SPANGENBERG et al., see section 3f

95 089
G. SUBRAMANIAN, CH. SRIDEVI NAIDU, Gautam MISHRA, Varadaraj BHAT, N. UDUPA* (*College of Pharmacy, Manipal, Karnataka, India): Stability indicating HPTLC determination of oxcarbazepine in tablets. 56th IPC 2004, Abstract No. GP-47. Stability indicating HPTLC determination of oxcarbazepine in tablet dosage form on silica gel with toluene - methanol 4:1. The Rf value of oxcarbazepine was 0.17. Quantitative determination by scanning at 255 nm. The compound was subjected to acid and alkali hydrolysis, oxidation, dry heat, and photo degradation. All degraded products were well resolved from the pure drug. The method was validated for accuracy, precision, linearity, robustness, and recovery. Pharmaceutical research, quality control, postchromatographic derivatization, comparison of methods, quantitative analysis, densitometry, HPTLC, oxcarbazepine 32a

95 090
A. SUGANTHI VIPIN PRAKASH*, Sapna SHRIKUMAR, K. A. Mirkasim, T. K. RAVI (*College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore 6

41 044
, India): HPTLC method for the simultaneous estimation of valdecoxib and tizanidine hydrochloride in tablets. IPC 56th 2004, Abstract No. GP-3. Simultaneous HPTLC determination of valdecoxib and tizanidine in tablets on silica gel with n-butyl acetate - formic acid - chloroform 7:3:2. Quantitative determination by densitometric scanning at 283 nm. The Rf values of valdecoxib and tizanidine were 0.78 and 0.39 respectively. Linearity range was 200 - 1000 ng/spot and 60 - 300 ng/spot respectively. Mean recovery for both of the compounds was 99.57 - 101.28 %. The method was validated for accuracy, precision, linearity, LOD, and LOQ. Pharmaceutical research, quality control, densitometry, quantitative analysis, comparison of methods, postchromatographic derivatization, HPTLC, valdecoxib, tizanidine 32a

95 093
S. TAMBE, S. KALE, P. SHAH, S. CHHAJED* (*M.G.V's Pharmacy College, Panchavati, Nasik, India): HPTLC analysis of beta-carotene in oral solid dosage forms. IPC 56th 2004, Abstract No. CP-32. A stability indicating HPTLC method has been developed for the analysis of solid dosage forms containing beta-carotene. HPTLC of beta-carotene on silica gel with petrol ether (40-60 °C) - methanol - toluene 4:8:1. Rf value of beta-carotene was 0.65-0.70. Quantification by densitometric evaluation at 460 nm. The method was validated for accuracy, precision, linearity, and stability, and can be adopted for routine analysis of beta-carotene in formulations. Pharmaceutical research, quality control, densitometry, comparison of methods, postchromatographic derivatization, quantitative analysis, beta-carotene 32a

95 094
S. TAMBE, S. KALE, S. KULKARNI*, S. CHHAJED (*M.G.V's Pharmacy College, Panchavati, Nasik, India): HPTLC analysis of ondansetron in oral solid dosage forms. IPC 56th 2004, Abstract No. GP-15. Stability indicating HPTLC determination of ondansetron in solid oral dosage forms on silica gel with chloroform - methanol 4:1. Quantitative determination by scanning at 310 nm. The Rf value was 0.62 - 0.64, linearity was 40 - 120 ng. The average recovery was 100.01 %. The method was found suitable for routine analysis of formulations containing ondansetron. Pharmaceutical research, quality control, densitometry, quantitative analysis, comparison of methods, postchromatographic derivatization, HPTLC, ondansetron 32a

95 034
J. D. VELICKOVIC et al., see section 23e

95 056
J. CHEN (Chen Jiatang)*, J. LIU (Liu Junyi), J. SU (Su Juan) (*Nanjing Tongrentang Pharm. Co., Ltd., Nanjing 210012, China): (Study of the quality standard for compound Yiqi granules) (Chinese). Chinese J. Trad. Pat. Med. (Zhongchengyao) 27 (2), 167-169 (2005). TLC on silica gel with 1) ethyl acetate - methyl ethyl ketone - formic acid - water 10:1:1:1; 2) chloroform - methanol 20:1; 3) chloroform - formic acid - water 13:7:2. Detection 1) under UV 254 nm; 2) by spraying with 10 % H2SO4 in ethanol and heating at 105 ¼C. Identification by fingerprint technique. Quantification of astragaloside IV by densitometry at 530 nm. Validation of the method by investigation of linearity (1.12 µg - 5.60 µg, r = 0.999); precision (RSD = 1.63 %, n= 5 within plate and RSD = 2.3 % plate to plate); reproducibility of five time assay towards the same sample (RSD = 3.12 %); standard addition recovery (97.69 %, RSD = 2.10 %, n = 5). The results for three real life samples are given. Pharmaceutical research, herbal, quality control, traditional medicine, qualitative identification, quantitative analysis, densitometry, astragaloside IV 32c

95 057
ZH. CHEN (Chen Zhongyi)*, T. YAO (Yao Tongwei), Y. PENG (Peng Yunzhen), ZH. ZHANG (Zhang Zhijian) (*Dep. Pharm. Sci., Zhejiang Univ., Hangzhou, Zhejiang 310006, China): (Assay and related impurity detection for magnesium fructose - diiphosphate) (Chinese). J. Chinese Pharm. Anal. 25 (1), 86-90 (2005). TLC of fructose, fructose-6-phosphate and related impurities on silica gel - carboxy methyl cellulose (CMC) -Na phase with n-butanol - acetone - glacial acetic acid - ammonia - water 35:15:20:3:27. Detection by spraying with 1 % sodium periodate solution followed by spraying with a solution of benzidine - ethanol - acetone - hydrochloric acid - water 0.8 g:80 mL:30 mL:1.5 mL:70 mL. Identification by fingerprint technique. Quantification by comparison with standards. The detection limits were investigated. In addition, the content of magnesium fructose diphosphate was determined by diphenylamine colorimetric method, and the related impurities are determined with the phosphomolybdic acid colorimetric method. Pharmaceutical research, herbal, quality control, qualitative identification, quantitative analysis, magnesium fructose, diphosphate, diphenylamine colorimetric method 32c

95 058
J. CUI (Cui Jiucheng)*, X. SONG (Song Xiaomei), Y. CAI (Cai Yan) (*Shanxi Coll. TCM, Xianyang, Shanxi, 712083, China): (Analysis of the processing principle of Fructus Schisandrae Sphenantherae by steaming with wine) (Chinese). Chinese J. Trad. Pat. Med. (Zhongchengyao) 27 (2), 176-178 (2005). HPTLC on silica gel with 1) cyclohexane; 2) cyclohexane - ethyl acetate 9:1. Detection 1) by spraying with 5 % phosphomolybdic acid in ethanol; 2) by spraying with vanillin - conc. H2SO4 solution. Identification of volatile oil by fingerprint technique. Determination of total lignan content by spectrophotometry. Analysis of the processing principle by comparison of the contents of the volatile oil and lignans in the extracts obtained by using different processing procedures, and discussing of the optimal processing procedures. Pharmaceutical research, traditional medicine, quality control, qualitative identification, HPTLC, volatile oil 32c

95 062
J. GAO (Gao Jiarong)*, J. ZHANG (Zhang Junru) (*No.1 Affiliated Hosp., Anhui Coll. TCM, Anhui, Hefei 230031, China): (Study of the quality standard for Qieyou Tangjiang extract) (Chinese). Chinese J. Trad. Pat. Med. (Zhongchengyao) 27 (1), 94-96 (2005). HPTLC on silica gel with 1) n-butanol - glacial acid - water 19:5:5; 2) petroleum ether (30-60 ¼C) - formic acetate - formic acid 15:5:1; 3) chloroform - ethyl acetate - methanol - formic acid 200:25:50:1. Detection 1) by spraying with 0.5 % ninhydrin in ethanol; 2) under UV 365 nm; 3) by spraying with vanillin - H2SO4 solution and heating. Identification by fingerprint technique. Quantification of emodin by densitometry at 440 nm. Validation of the method by investigation of linearity (0.1 µg - 0.5 µg, r = 0.998); precision (RSD = 3.8 %, n = 15 within plate and RSD = 3.2 %, n = 5 plate to plate); reproducibility of five time assay towards the same sample (RSD = 4.4 %); standard addition recovery (99.5 %, RSD = 2.2 %, n = 5). The results for some real life samples are given. Pharmaceutical research, traditional medicine, quality control, densitometry, HPTLC, quantitative analysis, qualitative identification, emodin 32c

95 063
CH. GUO (Guo Changqiang)*, J. LIU (Liu Jinxing), M. ZHANG (Zhang Min), Y. LI (Li Yan), CH. ZHOU (Zhou Chuanguo) (*Shandong Acad. TCM, Jinan, Shangdong 250014, China): (Study of the quality standard for Yijing Bushen granules) (Chinese). Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (9), 716-719 (2004). TLC on silica gel with 1) ethyl acetate - chloroform - formic acid 3:2:1; 2) chloroform - methanol 7:2; 3) petroleum ether (60-90 ¼C) - ethyl acetate 7:3. Detection 1) under UV 365 nm; 2) by spraying with 10 % H2SO4 in ethanol and heating at 105 ¼C for 5 min; 3) by spraying with vanillin - H2SO4 solution. Identification by fingerprint technique. Quantification of icarrin by HPLC. Pharmaceutical research, traditional medicine, quality control, herbal, quantitative analysis, qualitative identification, icarrin 32c

95 064
J. GUO (Guo Jingqiang)*, R. NIU (Niu Ruijie), G. HUANG (Huang Guifen) (*Tianjin municip. Inst. Drug Cont., Tianjin 300070, China): (Determination of astragaloside in Zilongjin tablets by thin-layer chromatography) (Chinese). J. Chinese Trad. and Herb. Drugs (Zhongcaoyao), 36 (2), 222-224 (2005). TLC on silica gel with chloroform - methanol - water 70:35:4. Detection by spraying with 10 % H2SO4 in ethanol and heating at 105 °C for 5 min. Identification by fingerprint technique. Quantification by densitometry at 530 nm. Validation of the method by investigation of linearity (0.458 µg - 2.748 µg, r = 0.998); precision (RSD = 2.48 %, n = 5 within plate and RSD = 4.69 % plate to plate); reproducibility of five time assay towards the same sample (RSD = 2.41 %); and standard addition recovery (96.11 %, RSD = 1.91 %, n = 5). The results for real life samples are given. Pharmaceutical research, traditional medicine, quality control, herbal, doping, quantitative analysis, qualitative identification, densitometry, astragaloside 32c

95 065
W. GUO (Guo Wenping), X. BAI (Bai Xiaoshi)*, L. LI (Li Laixiu) (*Sanmenxia People's Hosp., Sanmenxia, Henan 472000, China): (Preparation of Tianma Toufengling capsules and study of its quality standard) (Chinese). Chinese J. Trad. Pat. Med. (Zhongchengyao) 27 (2), 246-248 (2005). TLC of extracts prepared by different processing technology on silica gel with 1) petroleum ether (60 - 90 ¼C) - chloroform - methanol 10:3:2; 2) chloroform - methanol 5:1; 3) benzene - glacial acetic acid 4:1. Detection 1) under UV 254 nm; 2) by spraying with 1 % vanillin - H2SO4 solution and heating at 105 ¼C. Identification by fingerprint technique. Quantification of gastrodine by HPLC. Discussion of using the procedures for monitoring the preparation process and the quality control of the medicine products. Pharmaceutical research, traditional medicine, quality control, herbal, quantitative analysis, qualitative identification, gastrodine 32c

95 067
SH. HU (Hu Shuangfeng) (Ningbo Municip. Inst. Drug Cont., Ningbo, Zhejiang 315040, China): (Differentiation and identification of Xanthium sibiricum Patr. seed and the phoney, Xanthium Spinosam L. seed) (Chinese). Chinese J. Hosp. Pharm. (Zhongguo Yiyuan yaoxue Zazhi) 25 (2), 185-187 (2005). HPTLC on silica gel with n-butanol - glacial acetic acid - water 4:1:5. Detection by exposing to ammonia vapors. Identification by fingerprint technique combined with morphological differentiation and UV spectra comparison. Pharmaceutical research, traditional medicine, quality control, qualitative identification, HPTLC, differentiation and identification 32c

95 072
CH. LI (Li Chuncheng), X. YANG (Yang Xinghao)*, J. CUI (Cui Jinghao, Y. WANG (Wang Yanfei), J. ZHU (Zhu Jia) (*Pharm. R & D Centre, Nanjing Normal Univ., Nanjing 210097, China): (Separation and purification of the active fraction of Sinisan powder with macroporous resins) (Chinese). Chinese J. Trad. Pat. Med. (Zhongchengyao) 27 (1), 84-87 (2005). TLC screening of Sinisan powder extracts, purified with macroporous resins, on silica gel with 1) n-butanol - ammonia - ethanol 7:3:1; 2) chloroform - methanol 7:1. Detection 1) by spraying with 5 % AlCl3 in ethanol 2) by spraying with 5 % vanillin - H2SO4 solution. Identification by fingerprint technique. Screening of purification conditions by evaluation of the content of the active principle, and yield of the purified products. Type HP20 macroporous resin has been concluded to be the optimum for active fraction of the recipe in purification efficiency. Herbal, pharmaceutical research, traditional medicine, quality control, qualitative identification, separation and purification 32c

95 073
C. LI (Li Cunman)*, L. LI (Li Lanfang), Q. ZHANG (Zhang Qinzeng) (*Hebei Provin. Acad. TCM, Shijiazhuang, Hebei 050021, China): (Study of the quality standard for complex Xiaojingtong capsules) (Chinese). Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (8), 631-634 (2004). TLC on silica gel with 1) n-hexane - ethyl acetate 9:1; 2) chloroform - diethyl ether 1:1; 3) nbutanol - ethyl acetate - water 4:1:5. Detection by 1) exposing to iodine vapors; 2) spraying with 10 % H2SO4 in ethanol and heating at 105 ¼C. Identification by fingerprint technique. Quantifi- cation of astragaloside by densitometry at 530 nm. Validation of the procedure by investigation of linearity (1.1 - 5.5 µg per spot), precision (RSD = 1.82 %, n = 5 within plate and 1.99 %, n = 5 plate to plate), repeatability by standard addition recovery (100.1 %, RSD = 1.59, n = 6), etc. The results are given for a group of real samples. Pharmaceutical research, traditional medicine, quality control, herbal, quantitative analysis, qualitative identification, astragaloside 32c

95 074
F. LI (Li Fengqin) (Puyang Municip. Inst. Drug Cont., Puyang, Henan 457000, China): (Identi- fication of the main components and the dosage optimization in Shujin Qiefeng capsules) (Chinese). Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (9), app.17-19 (2004). HPTLC on silica gel with 1) toluene - acetone - ethanol - ammonia 20:25:3:2; 2) n-hexane - ethyl acetate - glacial acetic acid 15:5:1; 3) n-hexane - ethyl acetate - ammonia 20:20:1. Detection 1) by spraying with 5 % potassium iodobismuthate solution; 2) by spraying with 1 % potassium permanganate in diluted sulfuric acid followed by heating at 120 ¼C, and under UV 360 nm. Identification by fingerprint technique. Determination of the content of aconitine by comparison with standard. Pharmaceutical research, traditional medicine, quality control, qualitative identification, HPTLC, aconitine 32c

95 076
Q. MENG (Meng Qing)*, H. LIANG (Liang Hanming), G. CHEN (Chen Gengfu), X. GUO (Guo Xiaoling), Y. FENG (Feng Yifan) (*Guangdong Coll. Pharm. Guangzhou 510224, China): (Study of the quality standard for Tongfeng Huadyting tincture) (Chinese). Chinese J. Trad. Pat. Med. (Zhongchengyao) 27 (2), 158-161 (2005). HPTLC on silica gel with 1) cyclo-hexane - ethyl acetate - methanol 4:5:1; 2) n-hexane - ethyl acetate 3:1; 3) n-hexane - ethyl acetate 9:1; 4) cyclohexane - ethyl acetate - diethylamine 45:20:3. Detection 1) under UV 365 nm; 2) by spraying with 10 % H2SO4 in ethanol and heating; 3) by spraying with diluted potassium iodobismuthate solution followed by spraying with sodium nitrite solution in ethanol. Identification by fingerprint technique. Semi-quantitative determination of aconitine by comparison with the standard. Quantification of strychnine by HPLC. The results for some real life samples are given. Pharmaceutical research, traditional medicine, quality control, HPTLC, densitometry, quantitative analysis, qualitative identification, aconitine, strychnine 32c

95 077
Q. PENG (Peng Qian)*, H. ZHAO (Zhao Hua), GUO ZHANG (Zhang Guozhu) (*Hanzhong municip. Inst. Drug Cont., Hanzhong, Shanxi 723000, China): (Pharmacognostic identification of Saruma henryi and differentiation of Asarum siebodii) (Chinese). J. Chinese Trad. and Herb. Drugs (Zhongcaoyao), 36 (2), 277-280 (2005). HPTLC on silica gel with toluene - ethyl acetate - water - formic acid 20:10:1:1. Detection under UV 365 nm. Identification of volatile oil by fingerprint technique, combined with microscopy and a chemical method. Quantification of aristolochic acid A by HPLC. Pharmaceutical research, traditional medicine, quality control, qualitative identification, HPTLC, aristolochic acid A 32c

95 084
L. SHEN (Shen Linni)*, H. YU (Yu Haihong), Y. ZHENG (Zheng Yan) (*Zhejiang Deqing County TCM Hosp., Deqing, Zhejiang 313200, China): (Determination of chlorogenic acid in Liyin tablets by thin-layer chromatography) (Chinese). Chinese J. Hosp. Pharm. (Zhongguo Yiyuan yaoxue Zazhi) 25 (1), 90-92 (2005). TLC on silica gel with chloroform - ethyl acetate - formic acid 2:2:1. Detection under UV light. Identification by fingerprint technique. Quantification by densitometry at 325 nm. Validation of the method by investigation of linearity (0.52 µg - 4.68 µg, r = 0.9992); precision (RSD = 2.20 %, n = 5); reproducibility of five time assay towards the same sample (RSD = 3.10 %); standard addition recovery (99.0 %, RSD = 0.26 %, n = 5). The results for three real life samples are given. Pharmaceutical research, traditional medicine, quality control, herbal, doping, quantitative analysis, qualitative identification, densitometry, chlorogenic acid 32c

95 095
J. TANG (Tang Jingwen) (Shanghai Shuangji Pharm. Co., Ltd., Shanghai 201319, China): (Study of the quality control for Kangmoling capsules) (Chinese). Chinese J. Trad. Pat. Med. (Zhongchengyao) 27 (2), 150-154 (2005). TLC on silica gel with 1) chloroform - methanol 15:2; 2) nhexane - ethyl acetate 9:1; 3) toluene - ethyl acetate - acetone - methanol 50:25:25:3. Detection 1) by spraying with 10 % phosphomolybdic acid and heating; 2) under UV 365 nm; 3) by exposing to acetic anhydride vapors and heating at 140 - 160 ¼C and under UV 365 nm. Identification by fingerprint technique. Quantification of flavone glycoside by HPLC. The results for three real life samples are given. Pharmaceutical research, traditional medicine, quality control, densitometry, quantitative analysis, qualitative identification, flavone glycoside 32c

95 096
X. WANG (Wang Xiaoling) (Luoyang Municip. Inst. Drug Cont., Luoyang, Henan 471003, China): (Identification of the medicinal herb rhubarb and its preparations by thin-layer chromatography) (Chinese). Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (9), app.19-20 (2004). HPTLC on silica gel with petroleum ether (30 - 60 ¼C) - formic acetate - formic acid 15:5:1, at 11 ¼C and humidity of 40 %. Detection by exposing to ammonia vapors. Identification by fingerprint technique and comparison with the standards. Pharmaceutical research, traditional medicine, quality control, qualitative identification, HPTLC, emodin 32c

95 097
M. XIN (Xin Meiyu) (Guangdong Wannianqing Pharm. Co., Ltd., Shantou, Guangdong 515031, China): (Study of the quality standard for Buxie Danggui extract) (Chinese). Chinese J. Trad. Pat. Med. (Zhongchengyao) 27 (2), 225-227 (2004). TLC on silica gel with 1) petroleum ether (30 - 60 ¼C) - ethyl acetate 9:1; 2) benzene - glacial acetic acid 4:1. Detection 1) by spraying with 1 % vanillin solution and heating at 105 ¼C for 10 min; 2) under UV 365 nm. Identification by fingerprint technique. Quantification of ferulic acid by densitometry at 325 nm. Validation of the method by investigation of linearity (0.16 µg - 1.6 µg, r = 0.9992); precision (RSD = 1.8 %, n= 5 within plate and RSD = 2.3 % plate to plate); reproducibility of five time assay towards the same sample (RSD = 0.5 %); and standard addition recovery (97.17 %, RSD = 0.9 %, n = 5). The results for three real life samples are given. Pharmaceutical research, traditional medicine, quality control, herbal, doping, quantitative analysis, qualitative identification, densitometry, ferulic acid 32c

95 098
J. ZHANG (Zhang Junping)*, X. HUANG (Huang Xiaolan), H. LE (Le Haiping) (*Nanchang Municip. Inst. Drug Cont., Nanchang, Jiangxi 330003, China): (Study of the quality control of Kangbingdu oral liquid) (Chinese). Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (9), App.12- 14 (2004). TLC on silica gel with 1) n-butanol - glacial - water 19:5:5; 2) chloroform - methanol - glacial acetic acid - 17:2:1; 3) two fold development with benzene - acetone 9:1. Detection 1) by spraying with ninhydrin solution and heating at 105 ¼C; 2) by spraying with 5 % vanillin solution and heating; 3) by spraying with a solution of 8 % vanillin in ethanol - H2SO4, and heating at 105 ¼C. Identification by fingerprint technique. Quantification of phyllyrin by densitometry at 280 nm. The quantitative procedure is validated by investigating its linearity (1 - 5 µg/spot, r = 0.9998); precision (RSD = 0.36 % n = 5); repeatability (RSD = 2.92 % n = 5) and standard addition recovery (99.6 %, RSD = 2.4 %, n = 5), etc. The determination results are given for a group of real life samples. Pharmaceutical research, traditional medicine, quality control, herbal, quantitative analysis, qualitative identification, densitometry, phyllyrin 32c

95 099
Y. ZHANG (Zhang Yujie)*, H. HUANG (Huang Haixin), H. Tian (Tian Hong) (*Nanyang Municip. Inst. Drug Cont., Nanyang, Henan 473061, China): (Study of the quality standard for Pingxiao capsules) (Chinese). Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (9), App.14-16 (2004). TLC on silica gel with 1) toluene - ethyl acetate - formic acid 25:20:4; 2) ethyl acetate - methanol - water 100:17:13. Detection 1) by spraying with diazotized para-nitroaniline solution; 2) by spraying with 5 % AlCl3 in ethanol and under UV 365 nm. Identification by fingerprint technique. Quantification of hesperidin by HPLC. Pharmaceutical research, traditional medicine, quality control, herbal, quantitative analysis, qualitative identification, hesperidin 32c

95 100
ZH. ZHAO (Zhao Zhi Qiang) (Shanghai Leiyunshang Pharm. Co., Ltd., Shanghai 201517, China): (Study of the quality standard for Funing granules) (Chinese). Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (9), App. 3-5 (2004). TLC on silica gel with 1) n-hexane - ethyl acetate 9:1; 2) chloroform - methanol 5:2; 3) n-hexane - ethyl acetate 3:1. Detection 1) under UV 365 nm; 2) by spraying with 10 % H2SO4 in ethanol and heating at 110 ¼C; 3) by spraying with 5 % FeCl3 in ethanol. Identification by fingerprint technique. Quantification of icariine by HPLC. Pharmaceutical research, traditional medicine, quality control, herbal, doping, quantitative analysis, qualitative identification, icariine 32c

95 101
L. ZHOU (Zhou Lingying)*, X. CAO (Cao Xiaolan), X. BAI (Bai Xiaochun) (*Sichuan Enwei Inst. TCM, Chengdu, Sichuan 610041, China): (Study of the quality standard for Jieeryin effervescent tablets) (Chinese). Chinese J. Trad. Pat. Med. (Zhongchengyao) 27 (1), 34-37 (2005). TLC on silica gel with 1) benzene - acetone - ethyl acetate - ammonia water 10:15:20:1; 2) ethyl acetate - butanone - formic acid - water 5:3:1:1; 3) n-butanol - glacial acetic acid - water 7:1:2. Detection 1) by spraying with potassium iodobismuthate solution; 2) by spraying with 2 % FeCl3 in ethanol; 3) by spraying with vanillin - H2SO4 solution and heating; 4) under UV light. Identification by fingerprint technique. Quantification of geniposide by HPLC. The results for ten real life samples are given. Pharmaceutical research, herbal, quality control, traditional medicine, qualitative identification, quantitative analysis, densitometry, geniposide 32c

95 052
S. BASAR, Angelika KOCH* (*Frohme-Apotheke, Frohmestrasse 14, 22457 Hamburg, Germany): Test of the stability of olibanum resins and extracts. J. Planar Chromatogr. 17, 479-482 (2004). HPTLC of beta-boswellic acid (BA), acetyl-beta-BA, keto-BA, and acetyl-keto-BA and ethanolic extracts of olibanum resin on silica gel with toluene - ethyl acetate - formic acid - heptane 80:20:3:10 in a twin trough chamber without chamber saturation. Quantitative determination by reflectance measurement at 245 and 285 nm. Also two dimensional development with the same mobile phase in the second direction. Quality control, densitometry, HPTLC, quantitative analysis 32e

95 054
Anne BLATTER*, E. REICH (*CAMAG Laboratory, Sonnenmattstr. 11, CH-4132 Muttenz, Switzerland): High performance thin-layer chromatographic analysis of aristolochic acid in Chinese drugs. J. Planar Chromatogr. 17, 355-359 (2004). HPTLC of aristolochic acid A, B, and C and numerous plant extracts on silica gel in a saturated twin-trough chamber using the upper phase of the mixture toluene - ethyl acetate - water - formic acid 20:10:1:1. Quantitative determination by fluorescence measurement at 366 nm after derivatization with tin(II) chloride reagent. The working range and linearity, LOD and LOQ (based on the calibration plot), and precision (n = 6), were validated with methods described by K. Ferenczi-Fodor et al., J. AOAC Int. 84 (2001) 1265-1276. The stability of the analyte during chromatography was established by two dimensional chromatography. The new method enables visual detection of the acids with certainty at very low levels (400 pg absolute of aristolochic acid A) in plant material and can therefore be used for screening Chinese drugs to ensure their safety on the basis of absence of aristolochic acid. Quality control, toxicology, HPTLC, quantitative analysis, densitometry, aristolochic acid 32e

95 012
G. HORVÁTH et al., see section 4e

95 075
Q. MA (Ma Quanming)*, SH. LI (Li Shengyou) (*Qinghai Provin. People's Hosp., Xining, Qinghai 810007, China): (Preparation and quality control of Complex Zhike capsules) (Chinese). Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (8), Append. 9-11 (2004). TLC on silica gel previously immersed in 0.5 % NaOH solution, developed with 1) ethyl acetate - methanol - water 100:17:13; and 2) with the upper phase of toluene - ethyl acetate - formic acid - water 20:10:1:1. Detection by spraying with AlCl3 solution and under UV 365 nm. Identification by fingerprint technique and comparison with the standard. Quantification of scutellarin by HPLC. Traditional medicine, quality control, pharmaceutical research, herbal, quantitative analysis, qualitative identification, scutellarin 32e

95 019
Z. MALES et al., see section 8a

95 092
R. T. SANE*, S. N. MENON, S. SHAILAJAN, K. K. JARIPATKE (*Ramnarain Ruia College, Matunga, Mumbai-19, India): High-performance thin-layer chromatographic analysis of Asteracantha longifolia Nees. for determination of pharmacokinetics. J. Planar Chromatogr. 17, 483- 485 (2004). HPTLC of plant and plasma extracts on prewashed silica gel with toluene - ethyl acetate - methanol 30:3:1 in a twin-trough chamber previously equilibrated with the mobile phase. Quantitative determination by fluorescence/reflectance measurement at 366 nm. Pharmaceutical research, traditional medicine, HPTLC 32e

95 048
E. A. ABOURASHED* (*Department of Pharmacognosy, College of Pharmacy, King Saud University, P. O. Box 2457, Riyadh Saudi Arabia 11451): Validation and application of an HPTLC method for the determination of parthenolide in feverfew herbal products. J. Planar Chromatogr. 17, 375-378 (2004). HPTLC of parthenolide and extracts of feverfew capsules on silica gel with ethyl acetate - n-hexane 3:2 in glass chambers presaturated for 30 min. Detection by dipping in p-anisaldehyde reagent and heating at 105 °C for 5 min, followed by immediate densitometric scanning at 543 nm. The method is precise with CV< 5%; calibration recovery of 101.12 +/- 4.11 % and overall accuracy of 101.14 +/- 4.47 %. The levels of parthenolide in the products analyzed ranged from 0.03 to 0.24 %. Herbal, quality control, traditional medicine, HPTLC, densitometry, quantitative analysis, feverfew, parthenolide 32g

95 001
J. QU et al., see section 1